Flow Cytometry

Flow cytometry training is an integral part of the Hematopathology rotation, which stresses a combined diagnostic approach that synthesizes morphologic, immunophenotypic, and molecular/cytogenetic information. Residents will be exposed to the principles of flow cytometric analysis of hematologic malignancies during their 3-month junior and 1-month senior Hematopathology rotations, respectively. In addition, a 1-month Flow Cytometry elective rotation is available to those interested in expanding their knowledge base and practical skills in this area.

The flow cytometry laboratory is a state-of the art facility, which supports the MCW Cancer Center and a busy bone marrow transplant service of the Division of Hematology/Oncology. The instrumentation consists of two Becton-Dickinson FACSCanto II flow cytometers, equipped with three lasers, which allows routine 8-color analysis of 2,300 leukemia/lymphoma cases per year. All three Hematopathology faculty have extensive expertise in the immunophenotypic analysis of hematologic neoplasms, and run a busy flow cytometry research program, in addition to their clinical responsibilities.