Molecular Diagnostic Services

Molecular Diagnostic Services

The CTRL offers clinical molecular diagnostic testing for routine patient care, clinical trials, and research. Molecular services include: nucleic acid extraction from tissues (fresh, frozen, blood, formalin fixed paraffin embedded), nucleic acid quantification, PCR, real time PCR, quantitative PCR, dideoxy sequencing, pyrosequencing, capillary electrophoresis, gel electrophoresis, digital electrophoresis, and microarray analysis (Agilent format).

The molecular activities are performed by a Ph.D. level scientist with approximately 27 years of research experience, of which 11 years are focused exclusively on molecular diagnostics.

  • Nucleic acid purification (Qiagen BioRobot EZ1)
  • Nucleic acid quantification (Nanodrop; Qbit)
  • Agilent Bioanalyzer
  • PCR (ABI Veriti)
  • Real time PCR (Lightcycler; ABI 7500)
  • Reverse transcriptase PCR
  • Quantitative PCR (Lightcycler; ABI 7500)
  • Dideoxy sequencing (ABI 3500)
  • Pyrosequencing (Biotage PSQ 96MA)
  • DNA fragment analysis (ABI 3500)
  • DNA microarray analysis (Innopsys Scanner)
  • Ozone-free working environment (SciGene)
  • Gene expression microarray analysis (Innopsys)
  • Gel electrophoresis (Agarose, acrylamide)
  • Fluorescence in situ hybridization (FISH)